They were rediscovered and translated by Rita Steblin in her book A History of Key Characteristics in the 18th and Early 19th Centuries in 1983. A German music theorist and composer John Mattheson laid it out in his writings way back in 1713, among many other related topics. Now, we didn't come up with these feelings all by ourselves. You have to create an atmosphere that conjures up their own experiences and memories.Īnd you do this by choosing the proper musical key that expresses the characteristics of those emotions. You have to touch people's hearts and minds. But you don't want to just add these words to your lyrics. Comprehending the complexities of combined emotionsīeing able to internalize all of these words and understand the deep richness they can conjure up can help expand the reach of the power of your music. They are subtle explanations of how specific base emotions combine to create more complex feelings. Unless you're going for some crazy juxtaposition effect, just find the feeling you want out of the list below and get to work! Understanding EmotionsĮveryone knows about the base emotions, and most people use certain other words as if they're synonyms for the base emotions. Well here's your list! We're going to help you match the tone and psychological effect that you want your song to have to a specific key so it's not a wasted effort. The key of patience, calmly waiting for fate, destiny, and the submission to providence and karma. Angry, Jealous, Fury, Despair, Burdened with negative energy. The Garment of Night, Surly, Blasphemous, Turning away the world and the divine. Optimistic and able to take control in order to ensure peace. Love, Clear Conscience, Hopeful Aspirations for the future and a better world. Belief in Heaven and reuniting with lost loved ones. Innocent Love, Satisfaction with the current state of affairs. A negative look at the experiences of life, competition, growth. Suffocation of the Heart, Lamentations, Life-Long Struggles. Putrefaction, Expansive viewpoints of a dark cosmos and existence. Worry of the future, of a failed plan, gnashing of teeth. Tearing at your hair and shirt, discontentment, long periods of lamentation and crying. Brilliant clarity of thought and feeling. Surmounting foes and finally finding rest in victory. F Minorĭeepest depression, lament over death and loss, groans of misery, ready to expire. Deeply angry but composed and sociable still. F MajorĬomplaisance, Controlled calmness over the readiness to explode. Like a princess locked in a tower longing for her rescuer and future lover. This key can carry grief, mournfulness, restlessness. Shouts of Joy, Complete Delight, yet Bickering, Short-fused, Ready to Fight. Quarrelsome, Boisterous, Incomplete Pleasure Love, Devotion, Intimacy, Openness, Honest Communion. Fear, hesitation, shuddering, goose bumps. D# Minorĭealing with anxiety and existential terror, deep distress, dark depression. Melancholy, feminine, brooding worries, contemplation of negativity. War marches, holiday songs, invitations to join the winning team. Screaming hallelujah's, rejoicing in conquering obstacles. It is capable of a laugh or smile to pacify those around, but the truth is in despair. An intimate conversation with God about recognition of wrongdoing and atonement. C# MinorĪ passionate expression of sorrow and deep grief. It is languishing and full of longing, a soul in search of something different. C Minorĭeclarations of love and lamenting lost love or unhappy relationships. There may be some contradictory emotions, showing that the key can be used to describe a range of polar topics. Also, b is used to denote flat while # is used to denote sharp. Musical Key Characteristicsīelow we list every commonly used key, major and minor, along with a succinct version of their emotive expression and also include a more verbose explanation so you can really dig deep for what you're looking to get across to your listeners.Ī quick way to find what you're looking for is to use your browser's find (search) function. After that, unless you're a super experienced musician you're going to rip the key off of some other song you know or you'll consult a list. I can almost guarantee you most songwriters think "Is this a positive emotion or a negative one?" If it's positive they know they're going to use a major key and if it's negative they'll use a minor key. It's not like professional songwriters choose a random key and slap some random chord changes in there and magically end up with the perfect feeling that matches the lyrics. Musical key characteristics are something that are never talked about for some odd reason.
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Share your picture for free or download a high quality image for a small fee and print it using Snapfish, Costo Photocenter, or a printing service of your choice. Step 2 Upload photos, choose a collage layout, arrange photos in the desired zones. VEED is an online video collage maker with lots of free. Our smart layout algorithm instantly builds a photo layout for you. How to Make Photo Collage Step 1 Upload the photo, which you need to edit and for the upload to complete. Looking for a quick and simple tool to make video collages Youve come to the right place. This photo collage maker is free to use and works on any device like iOS, Android, Windows, Google, and more. Simple steps to an amazing photo collage, picture grid, or a photo mosaic. Craft your own photo collage filled with memories, your favorite TV show, or a specific aesthetic you found inspiring from Pinterest. Then add audio, trim it, and set the volume. Step 2 Make a video Add more files using any of the methods from step 1. It provides a collage template that shows how your photos are. For complete instructions, read the guide. Aspose.Slides Collage Maker is a free online app for creating collages and merging pictures. Start making cover collages, header collages, banner collages, and more for Twitter, Facebook, and other platforms. Step 1 Upload files Choose the necessary files from any of your devices, or select them from Dropbox or Google Drive. Resize your photos to make a unified photo collage you can share on any social media platform, like Instagram, for your family and friends to see. With just a few clicks, you can create digital collages, scrapbooks, posters, invitations, calendars, and greeting cards. In Kapwing, you can edit, combine, and merge multiple pictures, videos, and music together to create the perfect photo collage. Picture Collage Maker Free is a simple-to-use Windows freeware that turns ordinary photos and pictures into stunning keepsakes to share with family and friends. Enhance your photos by adjusting the saturation, adding text, or adding a song that highlights the feeling inside the photos. Kapwing’s picture collage maker provides you with over 60 collage templates to choose from and still have the freedom to rearrange or resize your pictures. Combine your favorite pictures together in one photo collage or make your own mood board with different images to convey the color scheme or theme of another project you have. The downside, of course, is that it’s not the industry-standard like Photoshop is, meaning it wouldn’t be ideal if you’re pursuing graphic design as a career. Not only that, but it’s less demanding on your hardware than Photoshop is. Photoshop will saddle your wallet with their subscription pricing model, whereas Affinity Photo only needs to be purchased once. Much like Adobe Photoshop, Affinity Photo is used for editing photos.Īffinity Photo can do nearly all that Photoshop can, the primary difference being the price tag. Generally speaking, animation software is always going to set the bar higher when it comes to hardware requirements, which explains why it’s the most resource-hungry application on the list. It can also be used to create cartoons and animate characters. If you’d like to make simple animations with the vector graphics you’ve created in Illustrator, you can do so using Adobe After Effects - a design application intended for animation.Īfter Effects can be used to create lower thirds for videos - for example, those little “Subscribe” animations that pop up in the lower corner of a YouTube video you may be watching. They also make for crisp lines and sharp edges, making them ideal for designing logos, app icons, user interface, avatars, cartoons, and more.Īlthough Adobe Illustrator won’t be as demanding on your computer as Photoshop is, it’s still an Adobe application, meaning the bar is set relatively high as far as specs go. The benefit of working with vector graphics is that they can be scaled up infinitely without quality loss. Unlike raster images - which are made of pixels - vector graphics are made of coordinate points that dictate the properties of a graphic on an X and Y axis. Illustrator is the industry-standard when it comes to vector design. Seeing as how Adobe creative applications tend to be bloated and hog lots of system resources, it makes sense that a powerhouse like Photoshop sets the bar as high as it does when it comes to its hardware requirements. More specifically, Photoshop can do things like create realistic product mockups and design advertisements for online display campaigns. There’s no limit on what Photoshop is capable of. You can adjust the colors, add effects, paint and sketch, apply text, make distortions, and more. It allows you to edit photos and transform them in all sorts of unique and creative ways. Photoshop is Adobe’s flagship product, and it’s a brand in and of itself. Links associated with the application name will take you to the manufacturer’s documentation for up-to-date specifications: Application CPU Supported OS RAM GPU Disk Space Monitor Resolution Adobe Photoshop Intel or AMD 64-bit 2 GHz or faster Windows, MacOS 8 GB 1.5 GB 4 GB 1280 x 720 Adobe Illustrator Multicore Intel or AMD 64-bit Windows, MacOS 8 GB Not Required 2 GB 1024 x 768 Adobe After Effects Intel or AMD Quad-Core Processor Windows, MacOS 16 GB 2 GB 15 GB 1920 x 1080 Affinity Photo 64-bit Windows, MacOS 2 GB Not Required 953 MB 1280 x 786 Affinity Designer 64-bit Windows, MacOS 2 GB Not Required 934 MB 1280 x 786 PaintShop Pro Intel or AMD 1.5 GHz or faster Windows 2 GB Not Required 1 GB 1024 x 768 CorelDraw Intel Core i3/5/7 or AMD Athlon 64 Windows, MacOS 2 GB Not Required 1 GB 1280 x 720 GIMP Pentium 4 or AMD Athlon Windows, MacOS, Linux 256 MB Not Required 200 MB Not Specified Inkscape Not Specified Windows, MacOS, Linux Not Specified Not Required 500 MB Not Specified Krita Not Specified Windows, MacOS, Linux 4 GB Optional OpenGL 3.0 Not Specified Not Specified Graphic Design ApplicationsĬontinue on for more details on each application and how it can be used. The following information is relevant as of September 3rd, 2022. 1 Download Animal Crossing Pocket Camp for PC – Tested and Working Method.Have a look at the following tutorial to install Animal Crossing Pocket Camp for PC right away. To download Animal Crossing Pocket Camp for PC, you need an Android emulator like the BlueStacks or the BlueStacks 3. If you wish to get this game on your desktop PC or a Laptop, then this guide is all that you need for now.Īnimal Crossing Pocket Camp for PC can be played on a computer running Windows 7, Windows 8, Windows 8.1, Windows 10, MacOS and Mac OS X. The graphics of this game make it worth playing on a computer as well. To sum it up, it won’t be wrong to say that this game is all about living a life while camping and getting close this beautiful universe.Īnimal Crossing Pocket Camp is already out for Android platform. If there is some animal who eats fish, you will go fishing in the nearby pond and so on. If there’s an animal who eats fruits, you will grab some fruits from the trees for them. Some of these animals will be hungry and some will need a little bit of care to recover from illness. While you are enjoying the nature living in a camp, there will be animals needing your attention. If you are wondering why this game has the term Animal Crossing in its name, then let us enlighten you on that as well. You will find trees full of fruits, beautiful ponds full of fish and greenery everywhere around you. They have to install the camps and establish a complete living on the site. Players are going to play the role of the campsite manager. Upon choosing to explore the nature, the game begins and it takes you on the suitable camping areas. The game welcomes you with a question about what you would be doing if you get a vacation from your busy life. Animal Crossing Pocket Camp is set to take you on an exciting adventure. 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I have seen the thread on here asking about how guys are getting camper shells on these trucks while still being able to use the rambox and have seen the video with the gray ram 2500 using the topperezlift, which is what I want to use. Strut is 1 5/8 by 1/5/8" approx 2 feet long. I have a 20 with the rambox in the 5'7' bed. I also incorporated two washers between the aluminum bar and the U bracket to allow the strut to slide more freely. I then fabricated from 1.5" aluminum flat bar a spacer for the front side between the U bracket and the rail for stand off and spreading the load. Y-autopart Mounting Clamps Truck Caps Camper Shell Powder-Coated Replacement for Chevy Silverado Sierra 1500 2500 3500,Dodge Dakota Ram 1500 2500 3500,F150 F250,Toyota Tundra Set of 6 (Black) 605. MaxMate Soft Quad-Fold Truck Bed Tonneau Cover for 2009-2018 Dodge Ram 1500 2019-2022 Classic Only Fleetside 57 Bed (67) without Ram Box TCD484015. I removed the springs as they were not necessary. Here are the first pictures of the U bracket attached to the rail system and also a strut configured for the kayak rack.įor the U brackets, I used strut nuts (1/2") that I ground down to fit in the rail system. Snugtops all-new sleek and stylish REBEL, is the. Eventually, I will paint the kayak rack when it is finished. Snugtops all new XV (Xtra Vision) Truck Cap features an enlarged rear door and. Ram Trucks Rambox Ladder Truck rack for Ram Trucks with the factory Ram Boxes: The P3000 Aluminum system for the RAM RamBox has a weight capacity of 150. in through the tailgate gap and sidewalls, when using a truck bed cap or. Anyway, I will provide pics as I get those done. ESI Ultimate Tailgate Seal with Taper Seal Compatible with RAM 1500 with RamBox. I did get sidetracked as my wife wanted me to create a way for her to take her kayak by herself. My plan was to be able to use the same struts and just swap out the hardware for the top vs. I then started on the rack for my kayaks. 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A small moon made of milk or tied the planet, going through the center of the donut shaped world. So once upon a time, there was a planet shaped like a cheerio. What do you call a cow with all of its legs? High steaks.Ī cross eyed teacher couldn’t control his pupils. What do you call a cow with two legs? Lean beef. What do you call a cow with no legs? Ground beef. Why did one banana spy on the other? Because she was appealing. What do you call a beautiful pumpkin? GOURDgeous. Two birds are sitting on a perch and one says "Do you smell fish?"ĭo you know sign language? You should learn it, it’s pretty handy. I bet the person who created the door knocker won a Nobel prize. What do you do with a dead chemist? You barium. What do you call a girl with one leg that's shorter than the other? Ilene. The shovel was a ground breaking invention.Ī scarecrow says, "This job isn't for everyone, but hay, it's in my jeans."Ī Buddhist walks up to a hot dog stand and says, "Make me one with everything."ĭid you hear about the guy who lost the left side of his body? He's alright now. How was Rome split in two? With a pair of Ceasars. Why does Superman gets invited to dinners? Because he is a Supperhero. Have you ever heard of a music group called Cellophane? They mostly wrap. What did the Buffalo say to his little boy when he dropped him off at school? Bison. What cheese can never be yours? Nacho cheese. There’s a new type of broom out, it’s sweeping the nation. What did daddy spider say to baby spider? You spend too much time on the web. How much does a hipster weigh? An instagram. Why did the octopus beat the shark in a fight? Because it was well armed. My cat was just sick on the carpet, I don’t think it’s feline well. I cut my finger chopping cheese, but I think that I may have greater problems. and pulled a mussel.ĭo you know where you can get chicken broth in bulk? The stock market. How do you organize an outer space party? You planet. What do you call a belt with a watch on it? A waist of time. Why did the cookie cry? Because his father was a wafer so long! Want to hear a joke about paper? Nevermind it’s tearable. You can help bring back the bees by ordering cheerios’s free wildflower seeds and planting a garden home for bees, butterflies and wasps.What do you call a fake noodle? An Impasta. The reason why the bee is missing on the honey nut cheerios box is upsetting. Honey nut cheerios bee on scooter figure vintage 2001 general mill cereal prize. General Mills Relies On Honey For More Than Just The Honey In Honey Nut Cheerios The essence of cheerios also contains lanolin from sheep (or wool fat). You Can Also Avoid Using Pesticides And Leave Patches Of Land Bare For Critters To Thrive.īees don't screw in light bulbs. The company says 30 percent of the ingredients it uses depends on. * 3 grams of soluble fiber daily from whole grain oat foods, like honey nut cheerios cereal, in a diet low in saturated fat and cholesterol, may reduce the risk of heart disease. In Other Words, This Means That They Will Be Providing The Acreage Of About 3,000 Football Fields Full Of Essential Food Sources And Homes. The campaign goal was to give away 35 million wildflower seeds. Buzz also appeared as the host in the honey nut cheerios spelling bee game, which was named after the breakfast cereal. Nov 99 New York City 73Rd Annual Macy's Thanksgiving Day Parade. Hitting a bee hive or bixbee will bounce yellow jacket farther along. toronto star, mamove over kathleen wynne and glen murray. The world’s bee population is in crisis and honey nut cheerios is coming to the rescue, minus its perky mascot. Here are some expert tips from our partner: Honey nut cheerios has made a commitment that by the end of 2020, oat farms that supply oats for honey nut cheerios will host 3,300 total acres of dedicated pollinator habitats across 60,000 acres of land. Source: Tune in again soon, bugs and girls. In fact, honey nut cheerios is not vegan because its ingredient is honey, which comes from animals. Source: īuzz, also known as the honey nut cheerios bee, is the mascot the honey nut cheerios cereal by general mills. In other words, this means that they will be providing the acreage of about 3,000 football fields full of essential food sources and homes. He was first introduced in 1979, alongside the honey nut cheerios cereal. A joke uses the slang “honey” (woman), “nut” (sex) and “cheerio. In other words, it is designed to be ultimately slim, have even illumination, and be customizable to your design. The Custom LED Light Panel is the new innovative light source. 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There are plenty of good reasons to choose 2x4 LED flat panels for your location. Offering all the appeal of LED lighting, a 2x4 LED flat panel is a fixture that is flatter than typical recessed lighting and is mounted flush in drop-in ceilings for a clean, attractive fixture type. Many of these options are available with manufacturer warranties to ensure satisfaction for years to come. Also, when it comes to being able to dim flat panels be sure to check the specs on each fixture, but most offer a 0-10v dimming option. The color temperature and lighting that LED flat panels produce tends to be even and very easy on the eyes. Flat panel fixtures are wonderful for reduced suspended ceiling cavities. Most 2 x 4 LED flat panel light fixtures are not completely flat on the back side making room for the LED drivers usually about 3 inches thick but check each fixtures specs for the correct thickness. Look for the highest lumen to watt ratio to be sure to receive the least amount of energy usage for you and your application. These LED flat panel 2x4 drop ceiling fixtures come in a variety of different lumen outputs based on wattages. Every option we offer has been made with the highest standards of quality for a long-lasting lighting solution. We offer 2x4 LED flat panel light fixtures from the top names in the industry, including WareLight, for dependable performance and quality construction you can trust. Our inventory of 2x4 LED panels offers a range of selections to meet your specific location needs with ease and affordability. Lymphocyte apoptosis and caspase activity were analyzed by flow cytometry and fluorimetry, and residual 8-methoxypsoralen concentrations by liquid chromatography–mass spectrometry. Cells collected with a Spectra Optia apheresis system were suspended in plasma or physiological saline (NaCl) and incubated with 200 ng/ml versus 340 ng/ml photosensitizer before UVA irradiation (Macogenic G2 or UVA PIT system). Materials and MethodsĪll patients ( n = 13) included in this study received photopheresis as medically indicated. This study aimed to optimize treatment by varying the 8-MOP starting concentration and the cell suspension medium. Several factors influence the efficacy of ECP with the photosensitizer 8-methoxypsoralen (8-MOP) and ultraviolet light A (UVA). IL-21 acute myeloid leukemia autologous feeder cells chimeric antigen receptor-modified NK cells closed GMP-compliant Prodigy system interleukin-3 receptor subunit alpha (CD123).Extracorporeal photopheresis (ECP), an apheresis-based therapy for various immunological diseases, works mainly by inducing apoptosis in lymphocytes. Next steps are the integration of the manual expansion procedure in the fully integrated platform for a standardized GMP-compliant overall process in this closed system that also may include gene modification of NK cells to optimize target-specific antitumor activity. After the fully automated NK cell separation process on Prodigy, a new NK cell expansion protocol was generated that resulted in high numbers of NK cells with potent antitumor activity, which could be modified efficiently by novel third-generation, alpha-retroviral SIN vector constructs. In fluorescence imaging, specific interactions that initiated apoptotic processes in the AML target cells were detected between CAR NK cells and KG1a. In addition, CAR NK cells showed higher degranulation and enhanced secretion of tumor necrosis factor alpha, interferon gamma, and granzyme A and B. These anti-CD123 CAR-engineered NK cells demonstrated improved cytotoxicity against the CD123 pos AML cell line KG1a and primary AML blasts. NK cells used for CAR transduction showed the highest anti-CD123 CAR expression on day 3 after gene modification. Compared to freshly isolated NK cells, expanded NK cells expressed significantly higher levels of NKp30, NKp44, NKG2D, TRAIL, FasL, CD69, and CD137, and showed comparable cell viabilities and killing/degranulation activities against tumor and leukemic cell lines in vitro. Moreover, effector cell expansion in manually performed experiments with NK MACS ® containing IL-2 and irradiated autologous FCs and IL-21, both added at the initiation of the culture, induced an 85-fold NK cell expansion. Manually performed experiments to test different culture media demonstrated significantly higher NK cell expansion rates and an approximately equal distribution of CD56 dimCD16 pos and CD56 brightCD16 dim&neg NK subsets on day 14 with cells cultivated in NK MACS ® media. The process in its early phase of development led to a median T-cell depletion of log 3.5 after CD3 depletion and log 3.6 after the whole process, including CD3 depletion and CD56 enrichment steps. The Prodigy manufacturing process revealed high target cell viabilities (median 95.4%), adequate NK cell recovery (median 60.4%), and purity of 95.4% in regard to CD56 +CD3 - target cells. Cytotoxicity, degranulation, and cytokine release of transduced NK cells were determined against KG1a cells in flow cytometric analysis and fluorescent imaging. Additionally, expanded NK cells were gene modified to express chimeric antigen receptors (CARs) against CD123, a common marker for acute myeloid leukemia (AML). After medium optimization, beneficial effects for functionality and phenotype were investigated at the beginning of cell expansion with irradiated (25 Gy) autologous FCs at a ratio of 20:1 (feeder: NK) in the presence or absence of IL-21 (100 ng/mL). Enriched NK cells were adjusted to starting cell concentrations within approximately 1 × 10 6 effector cells/mL and cultured in comparative expansion experiments for 14 days with IL-2 (1,000 IU/mL) in different GMP-compliant media (X-VIVO ™10, CellGro ®, TexMACS ™, and NK MACS ®). Separation of primary human NK cells (CD56 +CD3 -) was carried out with the CliniMACS Prodigy ® in a single process, starting with approximately 1.2 × 10 9 leukocytes collected by small-scale lymphapheresis or from buffy coats. Therefore, previous expansion protocols for those effector cells were improved and optimized by using newly developed culture medium, interleukin (IL)-21, and autologous feeder cells (FCs). However, good manufacturing practice (GMP)-compliant manufacturing of clinical-grade NK cells at sufficiently high numbers represents a great challenge. The administration of ex vivo expanded natural killer (NK) cells as potential antitumor effector cells appears to be suitable for effector cell-based immunotherapies in high-risk cancer patients. |
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